Figure 4: Summary of results from HDX-MS experiments.
From: Mass spectrometry locates local and allosteric conformational changes that occur on cofactor binding
(a) Summed difference in deuterium uptake between Fdc1Ubix (top) and Fdc1 (bottom) from the N to the C terminus of a single Fdc1 unit. (b,c) The deuterium uptake mapped onto the structure of Fdc1Ubix (PDB ID: 4ZA4). (b) depicts two surface representations transformed through 180°, whereas (c) shows a single cartoon representation of secondary structure elements. Differences in deuterium uptake are coloured according to the key with the cofactor prFMN shown in purple. (d) shows the same exchange data mapped directly to the linear sequence, the residues that interact with the cofactor are outlined in purple, while the residues that are involved in Mn2+ binding are outlined in orange.
