Figure 7: EPO enhances macrophage migration.

(a,b) Peritoneal macrophages from WT (a), or EPOR-MKO (b) mice were incubated with different concentrations of rhEPO and their chemotaxis to ATP were analysed (n=4). (c) Isolation of mediastinal lymph nodes (MLNs) 4 h after i.p. Indian ink injection. (d,e) pHrodor-labelled ZymA (i.p., 1 mg per mouse) together with rhEPO (i.p.) or PBS was given to male WT mice. MLNs were collected at indicated intervals for analysis of pHrodor⁺F4/80⁺ cells by flow cytometry (n=3). (f,g) pHrodor-labelled ZymA (i.p., 1 mg per mouse) together with rhEPO (i.p.) or PBS was given to male EPOR-MKO or EPOR-C mice. MLNs were collected at indicated intervals for analysis of pHrodor⁺F4/80⁺ cells by flow cytometry (n=3). (h,i) WT or CGD mice were given rhEPO (i.p.) or PBS for 1 day before pHrodor-labelled zymA (i.p., 1 mg per mouse) and every 24 h thereafter. MLNs were collected at indicated intervals for analysis of pHrodor⁺F4/80⁺ cells by flow cytometry (n=3). Representative data from at least two independent experiments are shown. Error bars represent the s.e.m. *P<0.05, two-tailed unpaired Student’s t-test.