Figure 1: Postsynaptic dLRRK is required for homeostatic synaptic compensation at the Drosophila larval NMJ.
From: LRRK2 regulates retrograde synaptic compensation at the Drosophila neuromuscular junction
(a) Representative traces of mEJCs and EJCs from Control (MHC-Gal4/+), GluRIIAM/R OE (muscle overexpression of UAS-GluRIIAM/R: UAS-GluRIIAM/R /+; MHC-Gal4/+), GluRIIAM/R OE; dLrrk heterozygous mutants (UAS-GluRIIAM/R/+; MHC-Gal4/dLrrke03680). (b) Quantification of mEJC, EJC and QC from the genotypes shown in a and dLrrk heterozygotes. n=21, 27, 19 and 19. (c) Quantification of mEJC, EJC and QC from Control (w1118), GluRIIA−/− (GluRIIASP16/Df(2L)cl-h4) and GluRIIA−/−; dLrrk −/− (GluRIIASP16/Df(2L)cl-h4; dLrrke03680), and representative EJC traces. n=32, 20 and 16. (d) Representative traces of mEJCs and EJCs from control (24B-Gal4/+), GluRIIA mutants carrying the 24B-Gal4 muscle driver (Df(2L)cl-h4/ GluRIIASP16; 24B-Gal4/+) and GluRIIA mutants expressing dLrrk-RNAi in the muscle (Df(2L)cl-h4/ GluRIIASP16; 24B-Gal4/UAS-dLRRK-RNAi). UAS-Dcr-2 is also present in all combinations, except for control. (e) Quantification of mEJC, EJC and QC from the genotypes shown in d and in dLrrk-RNAi OE (UAS-Dcr-2/+; 24B-Gal4/UAS-dLRRK-RNAi). n=19, 20, 18 and 19. (f) Quantification of mEJC, EJC and QC from BG380 (BG380-Gal4/+; +/GluRIIASP16), BG380; GluRIIA−/− (BG380-Gal4/+; Df(2L)cl-h4/ GluRIIASP16) and BG380; GluRIIA−/−; dLRRK-RNAi (BG380-Gal4/+; Df(2L)cl-h4/ GluRIIASP16; +/UAS-dLRRK-RNAi). UAS-Dcr-2 is present in all combinations. n=17, 18 and 20. Error bars represent s.e.m. *P<0.05, **P<0.01 and ***P<0.001. See Supplementary Table 1.
