Figure 2: Postsynaptic overexpression of LRRK2 results in a retrograde enhancement in neurotransmitter release.
From: LRRK2 regulates retrograde synaptic compensation at the Drosophila neuromuscular junction
(a) Representative traces of mEJCs and EJCs from dLRRK control (UAS-dLRRK/+), muscle overexpression of dLRRK (G14-Gal4/UAS-dLRRK) and muscle overexpression of hLRRK2 (G14-Gal4/+; +/UAS-hLRRK2). EJCs are ten superimposed consecutive traces recorded at 0.5 Hz; mEJCs represent continuous recordings. For details of the number of recordings and statistics, see Supplementary Table 1. (b) Quantification of mEJC, EJC and QC from the genotypes shown in a and control larvae for hLRRK2 overexpression (UAS-hLRRK2/+). n=21, 20, 20 and 28. (c) Quantification of EJCs at 0.5, 1.0, 1.5 and 3 mM external Ca2+ concentrations for control (MHC-Gal4/+), n=20, 10, 12 and 13, and dLRRK OE (UAS-dLRRK/+; MHC-Gal4/+), n=20, 11, 13 and 15 NMJs. (d) Muscle 6/7 NMJs double-stained with anti-GluRIIC (red) and anti-HRP (blue) in control (MHC-Gal4/+) and larvae overexpressing dLRRK in the muscle (+/UAS-dLRRK; MHC-Gal4/+). Scale bar, 10 μm. (e) Quantification of total number of type 1 boutons and muscle surface area from the genotypes shown in d and larvae overexpressing hLRRK2 in muscle (MHC-Gal4/UAS-hLRRK2). n=10, 10 and 10 NMJs. (f) Quantification of mEJC, EJC and QC from MHC control (MHC-Gal4/+), dLRRKR1441C OE (UAS-dLRRKR1441C/+; MHC-Gal4/+) and dLRRKR1441G OE (MHC-Gal4/UAS-dLRRKR1441G). n=15, 18 and 18. See also Supplementary Table 1. (g) Quantification of mEJC, EJC and QC from MHC control (MHC-Gal4/+), hLRRK2 OE (MHC-Gal4/UAS-hLRRK2), hLRRK2I2020T OE (MHC-Gal4/UAS-hLRRK2I2020T) and hLRRK2G2019S OE (MHC-Gal4/UAS-hLRRK2G2019S). n=12, 13, 20 and 20. Error bars represent s.e.m. *P<0.05, **P<0.01 and ***P<0.001. See also Supplementary Table 1.
