Figure 4: Retrograde synaptic enhancement by dLRRK and hLRRK2 is dependent on translation.
From: LRRK2 regulates retrograde synaptic compensation at the Drosophila neuromuscular junction
(a) Representative traces of mEJCs and EJC from control (G14-Gal4/+), dLRRK muscle overexpression (G14-Gal4/UAS-dLRRK) and dLRRK muscle overexpression in eIF4E heterozygotes (G14-Gal4/UAS-dLRRK; eIF4Es058911/+). (b) Quantification of mEJC, EJC and QC from the genotypes shown in a, as well as hLRRK2 overexpression in the muscle (+/G14-Gal4; UAS-hLRRK2/+) and hLRRK2 muscle overexpression in eIF4E heterozygotes (+/G14-Gal4; UAS-hLRRK2/eIF4Es058911). n=13, 18, 16, 10 and 10. (c) Quantification of mEJC, EJC and QC from control larvae for dLRRK (UAS-dLRRK/+), larvae overexpressing dLRRK in muscle (G14-Gal4/UAS-dLRRK), dLRRK muscle overexpression in S6k heterozygotes (UAS-dLRRK/G14-Gal4; +/S6kl−1), control larvae for hLRRK2 (UAS-hLRRK2/+), hLRRK2 overexpression in muscle (G14-Gal4/+; +/UAS-hLRRK2) and hLRRK2 muscle overexpression in S6k heterozygotes (+/G14-Gal4; UAS-hLRRK2/ S6k1−1). n=21, 20, 20, 20, 28 and 20. (d) Quantification of mEJC, EJC and QC from larvae overexpressing dLRRK in muscle (MHC-Gal4/UAS-dLRRK) or in combination with Tor heterozygocity (UAS-dLRRK/MHC-Gal4; +/Tor). n=14 and 19. (e) Quantification of mEJC, EJC and QC from larvae overexpressing TOR in muscle (MHC-Gal4/UAS-TOR) or in combination with dLrrk heterozygocity (UAS-TOR/MHC-Gal4; +/dLrrke03680). n=16 and 19. (f) Quantification of mEJC, EJC and QC from control larvae (MHC-Gal4/+) and larvae overexpressing dLRRK in muscle (+/UAS-dLRRK; MHC-Gal4/+) grown on normal food or food supplemented with 500 mg ml−1 cycloheximide or 1 μM rapamycin for 12 h. n=10, 10, 10, 8 and 8. Error bars represent s.e.m. *P<0.05, **P<0.01 and ***P<0.001. See also Supplementary Table 1.
