Table 2 Carbon utilization phenotypes observed for pSymB deletion mutants.

Strain	Phenotypes
	Biolog	Both	Model
ΔB154	L-histidine	None	None
	D-glucosamine
ΔB141	Palatinose^*	D-trehalose	Cis-4-hydroxy-D-proline^†
	Maltitol^‡	Trans-4-hydroxy-L-proline
ΔB163	Dulcitol^*	D-psicose	None
	D-glucosamine
ΔB180	Uridine^*	L-leucine	D-galactosamine^§
	N-acetyl-D-galactosamine^*
	Arbutin^*
	D-raffinose
	Organic acids^\|\|
ΔB181	L-lysine^*	L-histidine	D-galactosamine^§
	Dulcitol^*	D-tagatose
	N-acetyl-D-galactosamine^*
	D-glucosamine
ΔB108	L-ornithine	None	None
	L-serine
	L-asparagine
	L-alanine
ΔB109	None	None	None
ΔB179	Glycerol	L-arabinose	None
	L-lactic acid	M-inositol
ΔB118	None	L-ornithine	None
		M-inositol
		L-arginine
ΔB182	Acetic acid	D-melibiose	Protocatechuate^†
	Asparagine^‡	L-malic acid
	α-methyl-D-galactoside^‡	D-raffinose Succinic acid
	Melibionic acid^‡	D,L-malate
	Bromosuccinate^‡	Fumaric acid L-aspartate
ΔB161	Dulcitol^*	D-melibiose	None
	α-methyl-D-galactoside^‡	D-raffinose α-D-lactose
	Melibionic acid^‡	Lactulose Methyl-β-D-galactoside

The ‘Biolog’ column indicates the phenotypes observed experimentally that are not seen in silico with the iGD1575 model, and vice versa for the ‘Model’ column. The ‘Both’ column lists the phenotypes observed both experimentally and in silico.
^*The model does not produce biomass with this compound.
^†This compound is not present in the PM1 or PM2A plates, but the phenotype is confirmed in the literature.
^‡The model does not include this compound.
^§This compound is not present in the PM1 or PM2A plates, and the phenotype has not been reported in the literature.
^||Includes all tested L-amino acids, gly–glu, ala–gly, gly–asp, L-lactic acid, acetic acid, pyruvic acid, methylpyruvic acid, melibionic acid and gamma-aminobutyric acid (GABA).

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