Figure 9: Modulation of miniature postsynaptic currents by electrical coupling in neocortical layer 1.
From: Electrical coupling regulates layer 1 interneuron microcircuit formation in the neocortex
(a) Representative traces of inward mEPSCs (red traces, holding potential of −60 mV) and outward mIPSCs (blue traces, holding potential of +10 mV) recorded in Ctrl-shRNA and Cx36-shRNA groups in the presence of tetrodotoxin (5 μM). (b–e) Histograms (b,d) and cumulative distributions (c,e) of mEPSC amplitudes (b,c) and frequencies (d,e). Both the peak amplitude and frequency of mEPSCs in Cx36-shRNA group were significantly higher than those in Ctrl-shRNA group. (f–i) Histograms (f,h) and cumulative distributions (g,i) of mIPSC amplitudes (f,g) and frequencies (h,i). The peak amplitude and frequency of mIPSCs showed no significant differences between Cx36-shRNA and Ctrl-shRNA groups (n=12 in Cx36-shRNA group, n=8 in Ctrl-shRNA group, three mice for each group). *P<0.05, ***P<0.001, n.s., P>0.05, not significant. Two-tailed paired t-test. Error bars represent mean±s.e.m.
