Figure 5: Modifying E^FIL and E^FIL/ADH partially normalizes EC dynamics.

(a–d) Number of overtakes (a), the average (b) and maximal time (c) during which a salt and pepper (S&P) pattern is maintained, and the time required to acquire a stable S&P pattern (d) for in silico WT (isWT) ECs in non-mosaic sprouts, in which PFKFB3 was pharmacologically blocked (isPFKFB3^PI) simulated by modifying E^FIL (blue), E^FIL/COR (red) and E^FIL/ADH (green) in VEGF levels 2-fold higher than normal (2 × nVEGF). The white and grey bars represent a simulated isWT sprout in normal VEGF (nVEGF) and 2 × nVEGF levels, respectively. The horizontal red and blue dotted lines show the particular values of an isWT sprout in normal and 2 × VEGF levels, respectively. ts: timestep. Data are mean±s.e.m.; n=50; NS, not significant, **P<0.01, ***P<0.001 versus isWT in 2 × nVEGF; Student’s t-test.