Figure 4: IMAPP-based profiling of intracellular protein–protein interactions using DiZHSeC. | Nature Communications

Figure 4: IMAPP-based profiling of intracellular protein–protein interactions using DiZHSeC.

From: Genetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label

Figure 4

(a) A general workflow for IMAPP strategy. (b) Venn diagrams illustrating the number of HdeA client proteins identified in three biological replicates by IMAPP analysis. (c) The 52 proteins identified in at least two of the three replicates in IMAPP analysis. Left: percentage of mis-assignment of cytosolic proteins as HdeA client proteins. Envelope proteins are coloured in pink and cytosolic proteins are coloured in grey. Right: The 50 assigned HdeA client proteins include 22 previously reported clients (coloured in purple) and 28 newly identified clients (coloured in green), respectively. IMAPP analysis: a protein was assigned as a ‘hit’ when the Mascot search identifies at least two unique peptides, including one crosslinking peptide with MS-label modification and another non-crosslinking peptide without MS-label.

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