Figure 3: Proinflammatory cytokine induction in human PMNs and PBMCs by S. aureus depends on PSMα and lipopeptide expression.
From: Toll-like receptor 2 activation depends on lipopeptide shedding by bacterial surfactants
Absence of PSMα or lgt genes abrogates the capacity of S. aureus to stimulate TNF-α, IL-6 or IL-1β (a–c) and a USA300 mutant lacking PSMs plus Lgt requires complementation with both, PSM genes and lgt to regain the capacity to stimulate the release of these cytokines in human PBMCs (d–e) or IL-8 by human PMNs (f). Data represent means +/− s.e.m. of at least three independent experiments. *P<0.05, **P<0.01; ***P<0.001; ****P<0.0001; significantly different versus USA300 wild-type (a–c) or as indicated (d–f) as calculated by Student’s t-test.
