Figure 6: Lipoproteins can be released from S. aureus by incubation with synthetic PSMα peptides.
From: Toll-like receptor 2 activation depends on lipopeptide shedding by bacterial surfactants
Incubation of S. aureus USA300 PSM mutants with synthetic PSMα peptides leads to a strong increase in free His-tagged SitC detectable in western blots of culture filtrates (a, Supplementary Fig. 7) and restores the capacity of PSM mutant culture filtrates to stimulate IL-8 production in HEK-TLR2 cells (b). The synthetic lipopeptide Pam2CSK4 and TSB medium or (dimethylsulphoxide) DMSO at concentrations used to dissolve synthetic PSMs were used as positive and negative controls, respectively. Data in (b) represent means +/− s.e.m. of at least three independent experiments. **P<0.01; ****P<0.0001, significantly different versus USA300 Δα (b) as calculated by Student’s t test.
