Figure 3: Ion selectivity of AvGluR1.

(a) Current–voltage relationship of AvGluR1 in Na⁺ (solid circles) and K⁺ (open circles) extracellular solution. The reversal potential shifts by 19.7 mV from −24.3 to −2.5 mV. Inset: Representative Glu-gated currents at −100, −80, −60, −40, −20 to 0 mV in Na⁺ extracellular solution. (b, c) Reversal potentials (b) and current amplitudes (c) in normal extracellular ND96 solution (containing 1.8-mM Ca²⁺), Ca²⁺-free ND96 (zero added extracellular Ca²⁺ with 0.5-mM EGTA) and Cl⁻-free ND96 (Cl⁻ is replaced with gluconate). In (b) and (c) number of observations shown in parentheses and no statistically significant differences were observed using Student's t-test (P≥0.4).