Figure 1: Disruption of CINAP results in embryonic lethality.
From: The ATPase hCINAP regulates 18S rRNA processing and is essential for embryogenesis and tumour growth
(a) Schematic diagram of the CINAP locus and the targeting construct. The targeting vector replaced exons 3 and 4 with a neomycin-selectable marker flanked by LoxP sites. (b) CINAPflp/flp mice (8 weeks of age, female, n=2) were crossed with X-linked CMV-Cre mice (BALB/c, 8 weeks of age, male, n=1) to generate CINAP-deleted mice. (c) Observed and excepted birth ratio from CINAP+/− intercrosses (n=116). (d) Quantitative PCR analysis of CINAP expression in organs from adult CINAP+/+ and CINAP+/− mice (female, 8 week of age, n=3 for each genotype, randomly selected). Results are presented as mean±s.d. Killing of mice was performed according to the ethical guidelines approved by Peking University Laboratory Animal Center.
