Figure 2: Absence of ANGPTL4 attenuates the progression of atherosclerosis.

(a) Representative histological analysis of cross-sections of the aortic sinus from Ldlr^−/− and Angptl4^−/−Ldlr^−/− mice stained with H&E, Oil Red O and CD68/SMC-actin/DAPI. Quantification of the lesion area and macrophage content is shown in the right panels (n=9 per group). Scale bars, 400 μm. (b) Representative pictures of whole body Ldlr^−/− and Angptl4^−/−Ldlr^−/− mice on WD diet 12 weeks. (c) Body weight analysis of Ldlr^−/− and Angptl4^−/−Ldlr^−/− mice before and after WD diet (n=9 per group). (d) Representative histological analyses of cross-section of the ileum from Ldlr^−/− and Angptl4^−/−Ldlr^−/− mice stained with H&E and macrophage marker CD68. Scale bars, 400 μm. (e–h) Measurement of plasma TG (e), cholesterol (f) and lipoprotein profile from pooled plasma (g-h) of Ldlr^−/− and Angptl4^−/−Ldlr^−/− mice before and after 12 weeks on WD diet (n=9 per group). All data are the mean±s.e.m.; *P<0.05 by comparison with data from Ldlr^−/− mice by unpaired t-test.