Figure 3: Haematopoietic ANGPTL4 deficiency enhances atherogenesis.

(a,b) Representative histological analysis of cross-sections of the aortic sinus (a) and brachiocephalic arteries (b) isolated from male Ldlr^−/− chimeras with WT or Angptl4^−/− BM after 12 weeks of WD stained with H&E and Oil Red O. Quantification of lesion area and Oil Red O-positive lesion area is shown in the panel below respective figure (n=10 per group for aortic sinus and n=6 per group for brachiocephalic arteries). Scale bars, 400 μm. (c) Representative pictures from en face analysis of aortas from Ldlr^−/− chimeras with WT or Angptl4^−/− BM after 12 weeks on WD diet. Proportion of Oil Red O-positive area in the en face preparation is quantified in the lower panel (n=8 per group). Total cholesterol level (d), HDL cholesterol level (e) and TG level (f) in the blood plasma of Ldlr^−/− chimeras with WT or Angptl4^−/− BM before and after 12 weeks on WD (n=10 per group). (g) Lipoprotein profile from pooled plasma (n=5 each group) of Ldlr^−/− chimeras with WT or Angptl4^−/− BM after 12 weeks on WD diet. All data are the mean±s.e.m.; *P<0.05 by comparison with data from Ldlr^−/− chimeras with WT BM by unpaired t-test.