Figure 3: Radiation sensitive residues around the retinal Schiff base link. | Nature Communications

Figure 3: Radiation sensitive residues around the retinal Schiff base link.

From: Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Figure 3

The region is critical because proton pumping by bR requires light-driven trans–cis isomerization of retinal, rearrangement of water molecules (W401, W402 and W406) and changes of Asp85 and Asp212. Refinement of our SFX data (bRdark) results in well-resolved electron density (purple, 2Fo−Fc, 1 σ) for amino-acid side chains involved in this mechanism. Omission of water molecules results into strong positive difference peaks (green, Fo−Fc, 2.5 σ) indicating well-ordered structural water molecules.

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