Figure 3: Lineage switch phenotype is not detectable in parental leukaemia and is stable when CAR pressure is removed. | Nature Communications

Figure 3: Lineage switch phenotype is not detectable in parental leukaemia and is stable when CAR pressure is removed.

From: CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity

Figure 3

(a) Primary E2a:PBX ALL was single-cell cloned by limiting dilution, expanded and analysed by flow cytometry. Heatmap of cell surface marker expression evaluated by flow cytometry. (b) CD45.2+ B220+ leukaemic blasts from sample 59–61 obtained at day 58 following CAR demonstrating intermediate B/myeloid phenotype expressing for CD22, CD11b and Gr1, but negative for CD19. (c–e) In vivo passage of post-CAR CD19− relapsed leukaemia, with E2a:PBX control, in absence of additional CAR treatment. (c) B/Myeloid biphenotypic sample 25–2 passaged twice off pressure, with re-emergence of a CD19+ population in 16/21 mice in 3rd passage (E2a:PBX control n=16, 2nd n=10, 3rd n=12). (d) Lineage switch samples 24–6 (n=5 per group) and (e) A001 (E2a:PBX control n=3, 2nd n=3, 3rd n=4) passaged twice. Respective survival curves for 1 of 2 experiments shown on the right.

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