Figure 1: Expression patterns of SALM4 mRNAs and proteins.
From: SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3–LAR adhesion
(a) Distribution patterns of SALM4 mRNAs in mouse embryonic (E16 and E18) sagittal sections and postnatal brain (P7, P14, P21 and P56) horizontal sections, as revealed by in situ hybridization. E, embryonic day; P, postnatal day. Scale bar, 6 mm. (b) SALM4 antibodies specifically recognize SALM4 but not other SALM family proteins. C terminally Myc-tagged SALM family proteins (SALMs 1–5) expressed in HEK293T cells were immunoblotted with SALM4 antibodies (1820 antibody). (c) Tissue distribution of SALM4 proteins (1820 antibody). (d) SALM4 protein expression increases during postnatal rat brain development (1820 antibody). (e) SALM4 protein distribution in rat brain fractions (1820 antibody). H, homogenates; P1, cells and nucleus-enriched pellet; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. (f) Detection of SALM4 proteins in PSD fractions (2620 antibody). PSD-95 and synaptophysin (SynPhy) were used as controls. Note that the prestained markers here seem to migrate slightly faster than normal markers in other panels.
