Figure 4: SALM4 cis-interacts with SALMs 2/3/5.
From: SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3–LAR adhesion
(a) Enrichment of mature membrane proteins from forebrain crude synaptosomes (P21) using a WGA column. (b,c) The WGA eluants were immunoprecipitated (IP) with SALM4 antibodies (2026) and then subjected to preparative SDS–PAGE (c). A small aliquot of each precipitate was immunoblotted (IB) for SALM4 to confirm self-immunoprecipitation (b). The three bands (arrows) in c contained peptides of SALM4 (all three) and SALM2 (top blue). (d–f) SALM4 co-precipitates with SALM2/3/5 in heterologous cells. HEK293T cell lysates doubly transfected with SALM4 (Myc full-length, HA/Myc ectodomain or EGFP cytoplasmic domain)+EGFP-SALM2/3/5 (EGFP/Myc full-length) were IP and immunoblotted with the indicated antibodies. HA/Myc-SALM4 ectodomain represents SALM4 in the pDisplay vector tagged by both HA and Myc.
