Figure 3: Combination therapy-mediated MB49 tumour rejection is dependent on CD4+ and CD8+ T cells. | Nature Communications

Figure 3: Combination therapy-mediated MB49 tumour rejection is dependent on CD4+ and CD8+ T cells.

From: Interdependent IL-7 and IFN-γ signalling in T-cell controls tumour eradication by combined α-CTLA-4+α-PD-1 therapy

Figure 3

Isolated TILs from 13–15 days tumours, 1–3 days after the last treatment with α-CTLA-4, α-PD-1, combination of both (Combo) were analyzed by flow cytometry for (a) Total CD4+ T cells and CD8+ T cells. Representative flow panels are shown on the left and pooled results from five mice are depicted in bar graphs on the right. (b) Foxp3+ Treg in CD4+ T cells. Pooled results from five mice are shown in the right bar graph. (c) Ratios of Foxp3− to Foxp3+ in CD4+ T cells. (d) Ratios of CD8+ T cells to Foxp3+ CD4+ T cells. Mice depleted of CD4+ (GK1.5), CD8+ (2.43) or both (GK1.5+2.43) were treated with the combo and mouse survivals are presented (e). Data in the bar graphs are means±s.e.m. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 by one-way ANOVA with Bonferroni’s post hoc test. Data are representative of three independent experiments.

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