Figure 2: The tumour-suppressive effect of low molecular weight fraction from the L. casei ATCC334 culture supernatant.
From: Probiotic-derived ferrichrome inhibits colon cancer progression via JNK-mediated apoptosis
The tumour-suppressive effects of the L. casei ATCC334 culture supernatant fractions were examined after filtration through 3-, 5-, 10-, 30- or 50-kDa membranes (a). The tumour-suppressive fraction was dialyzed using a 0.5 kDa dialysis device (b). A tumour-suppressive effect was confirmed in the fractions that were separated from the L. casei ATCC334 culture supernatant by size-exclusion chromatography (c). The conditioned medium of L. casei was used as a positive control in the following study. The fractions were assessed using reverse-phase chromatography and a tumour-suppressive effect was identified in the 17th fraction by size-exclusion chromatography.
