Table 2 Single anthropogenic source regression results.

LD mtDNA	F _ST			nDNA	F _ST
Full data set	R ²	N >20 subset	R ²	Full data set	R ²	N >20 subset	R ²
RSG	0.4051***	RLA	0.6376***	SPV	0.0307	SPS	0.2915***
SPV	0.3785***	RSG	0.6288***	RLA	0.0235	DIST	0.0739*
RLA	0.3625***	SHB	0.6015***	DIST	0.0207	RSC	0.0459
SHB	0.3344***	RSA	0.5856***	RSG	0.0174	SPV	0.023
RSA	0.3334***	SPV	0.4004***	SHP	0.0157	SHB	0.0158
SHP	0.2629***	SHP	0.1844**	RSC	0.0121	RLA	0.0138
RSC	0.0137	RSC	0.0935*	SPS	0.0032	RSA	0.0134
SPS	0.0116	DIST	0.0759	SHB	0.0027	RSG	0.0112
DIST	0.0031	SPS	0.0504	RSA	0.0019	SHP	0.0019
LD mtDNA	D _EST			nDNA	D _EST
Full data set	R ²	N >20 subset	R ²	Full data set	R ²	N >20 subset	R ²
RSG	0.3653***	RSG	0.8710***	RSC	0.0273	RSC	0.1579**
RLA	0.3545***	RLA	0.8166***	DIST	0.0197	SPS	0.1199**
SPV	0.2922***	RSA	0.8138***	SPV	0.0121	DIST	0.0156
SHB	0.2523***	SHB	0.7661***	SHP	0.0078	SPV	0.0092
RSA	0.2450***	SPV	0.2689***	SPS	0.0071	SHP	0.0032
SHP	0.1802***	DIST	0.0337	RLA	0.0058	RLA	0.0027
SPS	0.0850**	RSC	0.0319	SHB	0.0040	SHB	0.0021
RSC	0.0306	SHP	0.0177	RSG	0.0030	RSG	0.0017
DIST	0.0000	SPS	0.0039	RSA	0.0008	RSA	0.0011

DIST, geographic distance; LD, low diversity; mtDNA, mitochondrial DNA; RLA, The Los Angeles River; RSA, The Santa Ana River; RSC, The Santa Clara River; RSG, The San Gabriel River; SHB, outfall for the Huntington Beach wastewater treatment facility; SHP, Hyperion sewage treatment plant; SPS, outfall for the Point Loma wastewater treatment plant; SPV, outfall for the Rancho Palos Verdes wastewater treatment plant.
R² values for single-variable matrix regression tests using the LD mtDNA and nDNA F_ST and D_EST values. Each measure of genetic structure was tested with the full data set and the n>20 data subset. *P<0.05, **P<0.01, and ***P<0.001.

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