Figure 5: Rac1 inhibition affects midbody-associated AMIS formation. | Nature Communications

Figure 5: Rac1 inhibition affects midbody-associated AMIS formation.

From: Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells

Figure 5

(a–d) MDCK cells were embedded in 3D Matrigel and allowed to grow for 24 h. Where indicated, cells were treated with 200 μM Rac1 inhibitor (b–d). Cells were then fixed and stained with phalloidin-Alexa594 (red) and anti-CGN (green) antibodies. White boxes indicate area zoomed in and shown in far right box. White arrows point to nascent lumen, black arrows point to areas of actin concentration. Consecutive images without an individual letter label show the same cell. (e,f) MDCK cells stably expressing GFP-CGN were embedded in 3D Matrigel. Untreated (e) or Rac1 inhibitor treated (f) cells were then imaged using time-lapse microscopy. In all cases, cell progression from metaphase to abscission was analysed. Arrow in (e) points to the midbody associated GFP-CGN.

Back to article page