Figure 9: Mutation of basic patch disrupts subcellular CGN targeting. | Nature Communications

Figure 9: Mutation of basic patch disrupts subcellular CGN targeting.

From: Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells

Figure 9

MDCK-CGN-KO cells were transfected with either wild-type CGN-GFP (a,b) or CGN-M1/M2-GFP mutant (R36/37/40/41A) (c–e). Cells were embedded in Matrigel, incubated for 12 h, fixed and stained with phalloidin-Alexa596. Arrows in (a) point to actin rich lumen surrounded by CGN-GFP-containing TJs. Arrows in (c) point to actin accumulation at the neck of bud-like structures. Arrows in (d) point to ectopic accumulation of actin and CGN rings. Finally, arrows in (e) point to intracellular formation of mini-lumens in CGN-KO cells.

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