Figure 3: USP17 shRNA alters cell morphology.
From: The deubiquitinating enzyme USP17 is essential for GTPase subcellular localization and cell motility
HeLa cells transfected with scrambled shRNA were left unstimulated (a) or stimulated with CXCL12 (100 ng ml−1) (b). USP17 shRNA1 cells were unstimulated (c) or stimulated with CXCL12 (100 ng ml−1) (d). Brightfield images were captured every 15 s for 10 min by time-lapse microscopy using Leica SP5. Representative images from triplicate experiments are shown. Scale bar, 20 μm. (e) RT–PCR was used to confirm USP17 depletion using mRNA from HeLa cells by examining USP17 and β-actin mRNA expression.
