Figure 4: USP17 depletion inhibits cytoskeleton rearrangements.
From: The deubiquitinating enzyme USP17 is essential for GTPase subcellular localization and cell motility
HeLa cells were transfected with scrambled shRNA or USP17 shRNA1, and stimulated with CXCL12 (100 ng ml−1) for 0, 2 or 5 min. (a) Cells were costained for actin-phalloidin (red), α-tubulin (green) and 4,6-diamidino-2-phenylindole (blue) and representative images captured by the Leica SP5 microscope. Arrows depict membrane ruffles; scale bar, 20 μm. (b) Quantification of membrane ruffles of triplicate experiments is represented graphically. Black bars are scrambled shRNA-, dark grey bars are USP17 shRNA1- and light grey are CYLD shRNA-transfected cells. Values are mean±s.e.m. ***P>0.005, *P>0.01; NS, not significant as determined by analysis of variance.
