Figure 5: Knockdown of USP17 inhibits GTPase localization and activation.
From: The deubiquitinating enzyme USP17 is essential for GTPase subcellular localization and cell motility
HeLa cells were transfected with scrambled shRNA or USP17 shRNA1 along with GFP-tagged Cdc42 (a), Rac1 (b) and RhoA (c). Cells were either left unstimulated or stimulated with CXCL12 (100 ng ml−1) for 5 min and captured by confocal microscopy (Leica SP5). Arrows indicate sites of plasma membrane localization. Scale bar, 20 μm. (d) Cells were transfected as in a–c, stimulated with CXCL12 (100 ng ml−1) for 0, 0.5, 2 or 5 min and analysed for GTPase activation by performing pulldown assays for the indicated GTPase. Pulldowns and whole-cell lysates were immunoblotted with Cdc42, Rac1 or RhoA antibodies as indicated. Data shown are representative of three experiments.
