Figure 7: USP17 depletion results in blunted downstream signalling.
From: The deubiquitinating enzyme USP17 is essential for GTPase subcellular localization and cell motility
HeLa cells were transfected with either scrambled shRNA or USP17 shRNA1 and stimulated with CXCL12 (100 ng ml−1) for 0, 0.5, 2 or 5 min. Protein was collected and whole-cell lysates were immunoblotted for pPAK, total PAK, active β1 integrin, total β1 integrin, pERK1/2 and total ERK1/2 as indicated.
