Figure 8: The phosphoproteome response to oxPt in CRC cells.

(a) A sequence logo was generated based on 205 detected phosphopeptides with potential ATM/ATR phosphorylation sites (pS/pTQ). (b) Fisher’s exact test on counts of dysregulated (log₂(ctrl+OX/ctrl)±0.58 and false discovery rate (FDR) ≤0.1) phosphopeptides revealed significantly increased upregulation of pS/pTQ motifs after oxPt treatment. (c) The number of altered phosphopeptides after 30 min of 16 μM oxPt treatment were counted and grouped into peptides with decreased phosphorylation (log₂(ctrl+OX/ctrl)<0.58) (‘Loss’) and increased phosphorylation (log₂(ctrl+OX/ctrl)>0.58) (‘Gain’). (d) KSEA was done on log₂(ctrl+OX/ctrl) ratios (as described in Fig. 5). Only substrate groups with indication of altered activities after oxPt exposure are shown (*P≤0.05, hypergeometric test). (e) Mean log₂ phosphorylation ratios for the nine substrate groups in d; (coloured boxes indicate P≤0.05, z-test). NS, not significant.