Figure 3: NOE is locus-independent.
From: Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
(a) NOE causes similar fold changes of editing at FANCF on-target and three known off-target sites16 in HEK293T cells. Cas9 was targeted to the FANCF locus with (dark blue, NOE) or without (light blue, RNP) non-homologous oligonucleotide and editing rates at FANCF or previously reported off-target (OT) loci were measured by NGS analysis. Data presented are the mean±s.d. of two biological replicates. *—denotes significant (P<0.05, Welch’s t-test) difference between RNP and NOE samples. Full editing rates are presented in Supplementary Fig. 7. (b,c) NOE increases editing rates ∼2.9-fold at the HEK293 site 1 and HEK293 site 3 known off-target sites, but cannot increase editing at the already saturated on-target site. Data presented as described in panel (a).
