Figure 6: Capture of early clonal expansion steps by single-cell-derived colony analysis.
From: Genetic hierarchy and temporal variegation in the clonal history of acute myeloid leukaemia
(a,b) Genotyping of single cell derived colonies from two de novo AMLs—UPN2014-027, (a) and UPN2015-027, (b)—with concomitant DNMT3A and TP53 mutations, as in Fig. 1. Losses of heterozygosity (LOH) were detected using informative SNPs. Asterisks indicate mutant allele detection or LOH. (c) Mutational patterns of individual colonies from patients with early initiating lesions. Cell sorting strategies before culture are indicated above each table. Dotted lines separate wild-type colonies, colonies with one mutation, and colonies with ⩾2 mutations/lesions. The number of colonies of each category is indicated below the tables.
