Figure 6: TGFβ signalling is active in LGR5+ve stem cells.
From: Inactivation of TGFβ receptors in stem cells drives cutaneous squamous cell carcinoma
(a) IHC analysis of PO4-SMAD3 (left panels) and Keratin 15 (right panels) in human normal skin. Insert shows strong PO4-SMAD3 staining in the telogen hair follicle Keratin 15+ve bulge stem cells. Scale bar, 100 μm. (b) IHC analysis of PO4-SMAD3 in murine normal skin. BG, bulge; DP, dermal papilla; IFE, inter-follicular epidermis; SG, sebaceous gland. (c) IHC analysis of LGR5-GFP in murine skin in the telogen phase of the hair cycle. Scale bar, 100 μm. (d) Immunofluorescence (IF) analysis of LGR5-GFP and PO4-SMAD3 in murine telogen skin. Nuclei are counterstained with DAPI. Scale bar, 50 μm. (e) qRT–PCR analysis of Tgfbr1, Tgfbr2, Tgfb1, Tgfb2, Tgfb3 and Smad7 in LGR5+ve (n=3 biological replicates) and LGR5−ve cells (n=3 biological replicates) freshly isolated from back skin in the telogen phase. Data are shown as ratios to the internal Gapdh control with error bars representing s.e.m. Statistical significance *P=0.04 (Mann–Whitney U-test, one-tailed test).
