Figure 7: Deletion of Tgfbr1 coupled with BRAF/KRAS activation leads to skin tumorigenesis.
From: Inactivation of TGFβ receptors in stem cells drives cutaneous squamous cell carcinoma
(a) Kaplan–Meier survival curve (left panel) of Lgr5CreER Tgfbr1fl/fl (n=12), Lgr5CreER BrafV600E(n=14), Lgr5CreER BrafV600E Tgfbr1fl/+ (n=23) and Lgr5CreER BrafV600E Tgfbr1fl/fl (n=26) mice (P≤0.0001 by log-rank, Mantel–Cox). Skin tumour-free survival curve (right panel) of Lgr5CreER BrafV600E (n=5), Lgr5CreER BrafV600E Tgfbr1fl/+ (n=10) and Lgr5CreER BrafV600E Tgfbr1fl/fl (n=19) mice. (b) Kaplan–Meier survival curve of Lgr5CreER KrasG12D (n=9) and Lgr5CreER KrasG12D Tgfbr1fl/fl (n=14) mice (P<0.0001 by log-rank, Mantel–Cox). (c) Macroscopic pictures of skin tumours from Lgr5CreER BrafV600E, Lgr5CreER BrafV600E Tgfbr1fl/+, Lgr5CreER BrafV600E Tgfbr1fl/fl and Lgr5CreER KrasG12D Tgfbr1fl/fl mice. (d) Representative staining of Keratin 1 (K1) and (e) Keratin 5 (K5) on Lgr5CreER BrafV600E, Lgr5CreER BrafV600E Tgfbr1fl/+, Lgr5CreER BrafV600E Tgfbr1fl/fl and Lgr5CreER KrasG12D Tgfbr1fl/fl mice. Scale bar, 100 μm.
