Figure 2: CYLD suppresses DNA damage-induced tumorigenesis independent of inflammation.
From: The tumour suppressor CYLD regulates the p53 DNA damage response
(a) CYLDΔ932IEC and CYLDΔ932FL male mice were given three 7-day cycles of 2% DSS in the drinking water. (b) Graphs showing body weight throughout the treatment and quantification of MEICS after each DSS cycle. (c) Representative H&E-stained distal colon sections from DSS-treated mice on day 180. Scale bar, 100 μm. One representative out of two independent experiments is shown. (d) CYLDΔ932IEC and CYLDΔ932FL female littermates received weekly injections of 10 mg kg−1 AOM for 6 weeks. (e) Representative endoscopic images on week 12. (f) Graph showing colon tumour numbers on week 18. (g) Representative H&E-stained histological images on week 18. Scale bar, 2,500, 50 μm. (h) Graphs showing the incidence of low-grade intraepithelial neoplasia (D1/D2) and high-grade intraepithelial neoplasia/carcinoma in situ (D3/Cais), and the cumulative size of high-grade intraepithelial neoplasia/carcinoma in situ D3/(Cais) in the indicated mice exposed to AOM. The evaluated samples comprise specimens from experiments shown in Fig. 2d-h. Pooled data from three independent experiments is shown. (i) 25 μg of DMBA was applied on the back of CYLDΔ932FL and CYLDΔ932epi female mice once a week during 8 weeks. (j) Graphs showing quantification of papilloma development on week 20. (k) Representative H&E-stained histological images of skin from the indicated mice on week 20. Scale bar, 500 and 200 μm. Pooled data from three independent experiments are shown. For all experiments data are shown as mean±s.d. Statistical significance was determined with Student’s t-test; ***P≤0.0005.
