Figure 4: p53 overexpression restores DNA damage-induced cell death and reduces tumorigenesis in CYLDΔ932^IEC mice.

Immunoblot analysis of p53, cleaved caspase-3 (CC3) and GAPDH (a), quantification of cleaved caspase-3-positive cells by FACS analysis (b) and qRT-PCR analysis of p53 target genes expression (c) in CpT-treated organoids from the indicated mice. Graphs in c show means of the absolute values±s.d. of biological triplicates. Statistical significance was determined with Student’s t-test; *P≤0.05, **P≤0.005, ***P≤0.0005. (d) Male littermate mice from the indicated mouse lines were injected with 7.5 mg kg⁻¹ AOM and 4 days later were given 2% DSS for 5 days. Graph shows colon tumour numbers on day 56. Pooled results from two different experiments are shown. (e) Female littermate mice from the indicated mice lines received weekly injections of 10 mg kg⁻¹ AOM for 6 weeks. Graph depicts colon tumour numbers on week 18. For the cancer experiment, data is shown as mean±s.d. Statistical significance was determined with Student’s t-test; P values are shown. qRT-PCR, quantitative real-time PCR.