Figure 6: CYLD and p53 interact in response to DNA damage.
From: The tumour suppressor CYLD regulates the p53 DNA damage response
(a) p53 immunoprecipitation from CpT-treated HEK-293 T cells transfected with the indicated plasmids. Immunoblot with anti-HA antibodies shows co-immunoprecipitated HA-CYLD. Expression levels of p53 and HA-CYLD in whole cell extracts (WCE) are shown. (b) HA-CYLD immunoprecipitation from CpT-treated HEK-293 T cells transfected with the indicated plasmids. Immunoblot with anti-GFP antibodies shows co-immunoprecipitated GFP-P53. Expression levels of GFP-p53 and HA-CYLD in WCE are shown. *corresponds to an IgG nonspecific band. (c) GST pull-down assay showing direct interaction between GST-p53 and His-CYLD. Coomassie staining shows equal amount of GST and GST-p53 proteins. Endogenous p53 was immunoprecipitated from primary keratinocytes (d) and HCT116 cells (e) treated with CpT for the indicated time points. Immunoblot with anti-CYLD antibodies shows the co-immunoprecipitated CYLD. Endogenous CYLD levels in WCE are shown.
