Figure 2: p53REs in the promoter regions of the genes for ISG15-conjugating system.
From: Positive feedback regulation of p53 transactivity by DNA damage-induced ISG15 modification
Deletions of the promoter regions in the ISG15 (a), UBE1L (b), UBCH8 (c) and EFP genes (d) were fused to a Luc reporter vector (pGL3) (left panels). The resulting vectors (P1–P4) were transfected to p53−/− HCT116 cells with pcDNA-HA-p53. p53+/+ HCT116 cells transfected with the same P1–P4 vectors were treated with and without ultraviolet (UV). p21-Luc was expressed in the cells as a control. These cells were then subjected to assay for the luciferase activity (right panels). The activity seen without any expression or treatment was expressed as 1.0 and the others were expressed as its relative values. Error bar, ±s.d. (n=3).
