Figure 7: Ectopic expression of VNUT in SDH neurons of Slc17a9−/− mice rescues tactile allodynia and enhances spinal ATP after PNI. | Nature Communications

Figure 7: Ectopic expression of VNUT in SDH neurons of Slc17a9−/− mice rescues tactile allodynia and enhances spinal ATP after PNI.

From: Dorsal horn neurons release extracellular ATP in a VNUT-dependent manner that underlies neuropathic pain

Figure 7

(a) PWT of WT mice before (pre) and after intraspinal injection of AAV-NSE-AcGFP or AAV-NSE-VNUT viral vectors without (w/o) PNI (n=6, two-way ANOVA with post hoc Bonferroni test). (b) Measurement of [ATP]e content in the ACSF media of spinal cord slices isolated from WT mice 28 days after intraspinal injection with AAV-NSE-AcGFP or AAV-NSE-VNUT viral vectors without PNI (n=13, Wilcoxon matched-pairs signed rank test). (c) Schematic diagram of experimental protocol for d,e. (d) PWT of Slc17a9−/− mice intraspinally injected with AAV-NSE-AcGFP or AAV-NSE-VNUT viral vectors before and after PNI (AAV-NSE-AcGFP; Slc17a9−/−: n=5, AAV-NSE-VNUT; Slc17a9−/−: n=6; *P<0.05, ***P<0.001 versus AAV-NSE-AcGFP;Slc17a9−/−, two-way ANOVA with post hoc Bonferroni test). (e) Measurement of [ATP]e content in the ACSF media of spinal cord slices isolated from Slc17a9−/− mice intraspinally injected with AAV-NSE-AcGFP or AAV-NSE-VNUT viral vectors 7 days after PNI (n=14, Wilcoxon matched-pairs signed rank test). Values are means±s.e.m. NS, not significant.

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