Table 1 Sequences remaining after each bioinformatic filtering step and taxonomic assignment.
From: Environmental DNA reveals that rivers are conveyer belts of biodiversity information
Sample name | Site name | Raw read count | (A) Merged | (B) Quality filter and trim | (C) Mapping | (D) Chimera removal | (E) Taxonomic assignment | Average %±s.d. of identical matches | Average±s.d. reference alignment length |
|---|---|---|---|---|---|---|---|---|---|
02-01_S12 | a | 859,156 | 496,181 | 389,859 | 251,400 | 250,725 | 32,673 | 97.7±2.7 | 204±85 |
08-01_S18 | ab | 754,781 | 479,497 | 433,506 | 151,744 | 151,422 | 21,989 | 94.1±2.7 | 195±77 |
07-01_S17 | b | 587,445 | 368,095 | 304,991 | 168,274 | 167,810 | 38,316 | 98.1±2.5 | 205±81 |
03-01_S13 | c | 458,635 | 294,565 | 247,769 | 144,850 | 144,453 | 37,659 | 97.9±2.6 | 213±85 |
01-01_S11 | cd | 770,055 | 512,041 | 411,234 | 176,874 | 176,231 | 36,509 | 94.9±2.8 | 195±74 |
04-01_S14 | d | 664,475 | 444,870 | 392,865 | 187,002 | 186,445 | 21,783 | 96.6±3.2 | 197±77 |
05-01_S15 | e | 673,495 | 364,002 | 330,412 | 172,045 | 171,389 | 20,376 | 95.8±3.4 | 192±76 |
06-01_S16 | f | 1,238,917 | 740,208 | 621,210 | 311,638 | 310,375 | 31,035 | 96.1±3.3 | 193±76 |
Total for samples | 6,006,959 | 3,699,459 | 3,131,846 | 1,563,827 | 1,558,850 | 240,340 | 96.6±3.2 | 200±80 | |
Mock c ommunity | 421,868 | 92,934 | 82,218 | 67,983 | 67,983 | 57,641 | 94.0±3.6 | 300.8±38 |
