Figure 4: OPTN regulates TNFR signalling complex I. | Nature Communications

Figure 4: OPTN regulates TNFR signalling complex I.

From: Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis

Figure 4

(a) Parental and OPTN-KO HeLa cells were stimulated with FLAG-TNF-α for the indicated times and then TNFR complex I was immunoprecipitated using an anti-FLAG antibody, followed by immunoblotting using the indicated antibodies. (b) Association of endogenous OPTN with free and/or conjugated linear ubiquitin chain on TNF-α stimulation. HEK293T cells were treated with FLAG-TNF-α and anti-OPTN immunoprecipitates were blotted with an anti-linear ubiquitin antibody. (c,d) Association of OPTN with RIP1 on TNF-α stimulation, examined at overexpressed (c) and endogenous (d) levels.

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