Figure 6: Differential BMP4 expression in GPCs versus MCPCs induced by Hedgehog signalling. | Nature Communications

Figure 6: Differential BMP4 expression in GPCs versus MCPCs induced by Hedgehog signalling.

From: Hedgehog–BMP signalling establishes dorsoventral patterning in lateral plate mesoderm to trigger gonadogenesis in chicken embryos

Figure 6

(a) SHH expression plasmid was electroporated into MCPCs at E2.0. (b,c) Transverse views of the mesonephros and presumptive gonad, corresponding to the boxed regions in a, at the untreated (control) side (b) or SHH-overexpressing side (c), stained for BMP4 mRNA. BMP4 expression was detected in the presumptive gonad (arrows in b) but not in the MCPCs (open arrowheads in b) on the untreated (control) side, but was detected in both MCPCs (white arrowheads in c) and GPCs (arrows in c) on the SHH-overexpressing side. (d) HipΔC22 expression plasmid was electroporated into the GPCs in E2.0 embryos. (e) Transverse views of the presumptive gonadal region, corresponding to the boxed regions in d. BMP4 expression was downregulated in the HipΔC22-electroporated side (open arrows) relative to the untreated (control) side (white arrows). MN: mesonephros; DA: dorsal aorta. Scale bars, 50 μm.

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