Figure 3: Structured illumination microscopy (SIM) analysis of paternal mitochondria surrounded by autophagosomes in zygotes.
From: Kinetics and specificity of paternal mitochondrial elimination in Caenorhabditis elegans
(a–f) Analysis of MTR-stained paternal mitochondria surrounded by LGG-1 autophagosomes in zygotes. In all panels, zygotes from mating of MTR-stained males and unstained hermaphrodites with the indicated genotypes right after sperm entry were stained with an antibody to LGG-1 as described in Fig. 2. Scale bar represents 5 μm. A higher magnification view of MTR-stained paternal mitochondria and LGG-1 autophagosomes is shown in the inset of each image panel. Scale bars in the insets are 1 μm. (g) Percentages of MTR-stained paternal mitochondria enclosed or partially surrounded by LGG-1 autophagosomes in the indicated crosses are shown. MTR-stained paternal mitochondria not in direct contact with any LGG-1 puncta were scored as a ‘none’ (not surrounded by an autophagosome). The significance of difference between different mating experiments was determined by Chi-square test. **P<0.01. ‘n.s.’ indicates no significant difference. The numbers of MTR-stained paternal mitochondria scored are 202 (a), 202 (b), 133 (c), 108 (d), 146 (e) and 130 (f), respectively. In all panels, fzo-1(tm1133) and drp-1(tm1108) alleles were used.
