Figure 6: miR-183/96/182 cluster decreases the expression of genes involved in plasticity and altered by NOR training, including HDAC9. | Nature Communications

Figure 6: miR-183/96/182 cluster decreases the expression of genes involved in plasticity and altered by NOR training, including HDAC9.

From: The microRNA cluster miR-183/96/182 contributes to long-term memory in a protein phosphatase 1-dependent manner

Figure 6

(a) Expression level of target genes in the hippocampus of miR-183/96/182 overexpressing mice: Cacnb4 (t6=5.42, **P<0.01), Gabra1 (t6=1.768, P=0.127), Prkcz (t6=3.675, *P<0.05), Nrg1 (t5=2.212, #P<0.1), Ppp2ca (t6=8.159, ***P<0.001), Grm5 (t5=3.246, *P<0.05), Gria1 (t6=3.261, *P=0.05), Usp13 (t6=2.98, *P=0.05), Hdac9 (t10=3.25, **P<0.01) and Nufip2 (t10=2.74, *P<0.05); controls, n=3–7; miR-183/96/182, n=4–5. (b) Top panel: Predicted target site of miR-182 seed sequence on HDAC9 3′ UTR. The seed sequence and its corresponding target sequence are highlighted in red (adapted from www.microrna.org); bottom panel: Relative luciferase activity (firefly luciferase (FL) to renilla luciferase (RL)) of HDAC9 3'UTR containing construct measured in N2A cells in the presence or absence of miR-182 mimic (t7=2.88, *P<0.05). (c) Representative blot (left panel) and quantification (right panel) of HDAC9 protein in mouse hippocampal extracts after NOR training (one-way ANOVA: F3,28=2.06, P=0.13; t-test: cage control versus NOR testing t(14)=2.05, #P<0.1, habituation versus NOR testing t(13)=2.24, *P<0.05; cage control, n=9, habituation, n=8, NOR training, n=8, NOR testing, n=7). Bar graphs represent mean±s.e.m.

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