Figure 4: mRNA profiling across AK/papilloma and cuSCC development.

(a) Correlation matrix of mRNAs differentially expressed in at least one signature in human samples shows that AKs span the spectrum of NS to cuSCC samples. (b) Unsupervised clustering of all genes across patient samples with complete sets (all three lesion types) demonstrates that in 6/8 sets, AK more closely resemble cuSCC. The Pearson correlation matrix is shown on top with the underlying heat map shown below. (c) A 70-gene signature of chromosomal instability derived from human cancers is highly enriched in AK and cuSCC to a similar degree, but not NS. (d) Correlation matrix of mRNAs differentially expressed in at least one signature in mouse samples demonstrates that PAPs much more closely resemble cuSCC than CHR.