Figure 1: Localization of the histone h4 mRNA on the 80S ribosome.
From: Ribosomal 18S rRNA base pairs with mRNA during eukaryotic translation initiation
(a) Overview of the h4/80S complex stalled in the pre-translocation state with h4 (red), eEF1A (red), A/T-site tRNA (magenta), P-site tRNA (green), 60S ribosomal subunit (blue) and 40S ribosomal subunit (orange). (b) h4 mRNA is inserted between the tip of ribosomal helix h16 (18S rRNA) and proteins uS3 and eS10. (c) Model of the h4 mRNA interactions with the apical loop of 18S rRNA helix h16. (d) Superimposition of eukaryotic and prokaryotic ribosomes highlighting the structural difference at the level of helix h16, creating a site in eukaryotes for mRNA binding (solid circle); eukaryote-specific protein eS30 in part takes the place of h16 in prokaryotes.
