Figure 4: The SH domain of CNOT1 directly interacts with YTHDF2. | Nature Communications

Figure 4: The SH domain of CNOT1 directly interacts with YTHDF2.

From: YTHDF2 destabilizes m6A-containing RNA through direct recruitment of the CCR4–NOT deadenylase complex

Figure 4

(a) Schematic diagram of human CNOT1 and the fragments used in b and c. (b,c) Interaction between YTHDF2 and CNOT1 fragments. HEK 293 cells were co-transfected with a plasmid encoding FLAG-tagged YTHDF2 and an HA-tagged CNOT1 fragment or a negative control (EGFP). Immunoprecipitation and immunoblotting were performed as in Fig. 3a–c. (d) Schematic diagram of human YTHDF2 and the fragments used in e and f. (e,f) Determination of a direct interaction between CNOT1-SH and YTHDF2 fragments by GST pull-down assays. Recombinant His-tagged YTHDF2 or its fragments and GST-tagged CNOT1-SH were purified, and equal amounts of both proteins were subjected to GST pull-down assay followed by SDS–PAGE separation and Coomassie Blue staining.

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