Figure 4: ATRA downregulates MLC-2 actomyosin contractility. | Nature Communications

Figure 4: ATRA downregulates MLC-2 actomyosin contractility.

From: ATRA mechanically reprograms pancreatic stellate cells to suppress matrix remodelling and inhibit cancer cell invasion

Figure 4

(a) Immunofluorescent images of total myosin light-chain 2 (MLC-2) and phosphorylated levels (pMLC-2). (b) Quantification of staining intensity (n>20 cells in all cases and three experimental replicates). Scale bar, 50 μm. (c,d) MLC-2 level expression at the protein (western blotting) and mRNA (qPCR) levels. All results are expressed as mean±s.e.m. and *P<0.05; **P<0.01; ***P<0.001; (t-test), three independent experiments for western blotting and qPCR.

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