Figure 2: DELLA expression patterns in M. truncatula roots inoculated with rhizobium and symbiotic nodules.
From: DELLA-mediated gibberellin signalling regulates Nod factor signalling and rhizobial infection
Histochemical analysis of GUS activity of MtDELLA transcriptional fusions in roots (1 day post inoculation (dpi) with S. meliloti) and nodules (21 dpi). Whole roots or 40 μm thick transversal root and nodule sagittal sections of MtDELLA1:GUS (a,b), MtDELLA2:GUS (d,e) and MtDELLA3:GUS (g,h) are shown. (c,f,i) are in situ hybridizations of MtDELLA1 (c), MtDELLA2 (f) and MtDELLA3 (i) transcripts in 40 μm thick nodule longitudinal sections. Root zones are described as follows: DZ, differentiation zone; EZ, elongation zone; PZ, proliferation zone. Nodule zones are described as follows: I, meristem; II, infection/differentiation zone; III, nitrogen-fixation zone (according to Vasse et al.7); R, root. Scale bars, 100 μm. One representative example out of n=20 independent roots for histochemical analysis of GUS activity and n=5 nodules for in situ hybridization is shown.
