Figure 1: A large-scale RNAi screen identifies MARCH1 as a repressor of insulin signalling.
From: MARCH1 regulates insulin sensitivity by controlling cell surface insulin receptor levels
(a) Schematic summary of the RNAi screen. (b) Relative cell number (n=3), calculated using Trypan blue exclusion, for HeLa cells expressing indicated shRNAs and grown in suboptimal insulin concentration (0.05 μg ml−1) for the indicated number of days. *compares NS and MARCH1 shRNAs at day 10. (c) Immunoblot analysis of AKT Ser473 phosphorylation in HeLa cells expressing either NS or MARCH1, MARCH9 or NHLRC1 shRNAs. (d) Relative 14C-2-deoxyglucose uptake (n=4) in HeLa cells expressing indicated shRNAs. *compares NS and indicated shRNAs in presence of insulin. (e) Relative glycogen synthesis (n=3) in HeLa cells expressing indicated shRNAs. *compares NS and indicated shRNAs in presence of insulin. In all panels, data are mean±s.e.m. and *P<0.05, comparisons by t-test. NS, nonsilencing.
