Figure 3: March1 knockdown improves insulin sensitivity in mice.
From: MARCH1 regulates insulin sensitivity by controlling cell surface insulin receptor levels
Male 12–20-week-old C57BL/6 J mice were treated with ASO for 2 weeks and fed regular chow or HFD as indicated. (a) Relative March1 mRNA expression in liver and WAT. (b,c) Plasma glucose (b) and insulin (c) excursions during ipGTT. (d) Plasma glucose and glucose-infusion rates during hyperinsulinemic-euglycemic clamp studies in mice fed regular chow or 3 weeks of HFD and treated with March1 ASO or control ASO. # denotes comparison of chow-fed groups and *denotes comparison of HFD-fed groups. (e) EGP during the basal period and during the steady-state period of the clamp. (f) Suppression of EGP during the clamp, expressed as % basal EGP. (g) Suppression of plasma NEFA concentrations during the clamp, expressed as % basal. (h) Whole-body glucose uptake during the clamp. Data are mean±s.e.m. In all panels, *P<0.05, **P<0.005, ##P<0.005, ***P<0.0005, ###P<0.0005. In a, n=3 mice per group; comparisons by two-tailed unpaired t-test. In b,c, n=5–8 mice per group; comparisons by two-way ANOVA. In d–h, n=9–11 mice per group; comparisons by two-way ANOVA. ANOVA, analysis of variance; ipGTT, intraperitoneal glucose tolerance tests.
